Creation of a Cellooligosaccharide-Assimilating Escherichia coli Strain by Displaying Active Beta-Glucosidase on the Cell Surface via a Novel Anchor Protein | Zendy

Tsutomu Tanaka | Zendy; Hitomi Kawabata | Zendy; Chiaki Ogino | Zendy; Akihiko Kondo | Zendy

Open Access

Creation of a Cellooligosaccharide-Assimilating Escherichia coli Strain by Displaying Active Beta-Glucosidase on the Cell Surface via a Novel Anchor Protein

Author(s) -

Tsutomu Tanaka,

Hitomi Kawabata,

Chiaki Ogino,

Akihiko Kondo

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00459-11

Subject(s) - escherichia coli , cellobiose , strain (injury) , enterobacteriaceae , escherichia coli proteins , chemistry , beta (programming language) , microbiology and biotechnology , biochemistry , biology , enzyme , gene , anatomy , cellulase , computer science , programming language

We demonstrated direct assimilation of cellooligosaccharide using Escherichia coli displaying beta-glucosidase (BGL). BGL from Thermobifida fusca YX (Tfu0937) was displayed on the E. coli cell surface using a novel anchor protein named Blc. This strain was grown successfully on 0.2% cellobiose, and the optical density at 600 nm (OD(600)) was 1.05 after 20 h.

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