Novel Multiplex Single Nucleotide Polymorphism-Based Method for Identifying Epidemic Clones of Listeria monocytogenes | Zendy

Sara Lomonaco | Zendy; Stephen J. Knabel | Zendy; Alessandra Dalmasso | Zendy; Tiziana Civera | Zendy; Maria Teresa Bottero | Zendy

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Novel Multiplex Single Nucleotide Polymorphism-Based Method for Identifying Epidemic Clones of Listeria monocytogenes

Author(s) -

Sara Lomonaco,

Stephen J. Knabel,

Alessandra Dalmasso,

Tiziana Civera,

Maria Teresa Bottero

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00429-11

Subject(s) - listeria monocytogenes , biology , single nucleotide polymorphism , multiplex , multiplex polymerase chain reaction , primer (cosmetics) , virulence , genetics , listeria , gene , polymerase chain reaction , microbiology and biotechnology , virology , genotype , bacteria , chemistry , organic chemistry

A novel primer extension-based, multiplex minisequencing assay targeting six highly informative single nucleotide polymorphisms (SNPs) in four virulence genes correctly identified and differentiated all four epidemic clones (ECs) of Listeria monocytogenes and 9 other strains initially misclassified as non-ECs. This assay allows rapid, accurate, and high-throughput screening for all known ECs of L. monocytogenes.

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