Pathotyping Escherichia coli by Using Miniaturized DNA Microarrays | Zendy

Muna F. Anjum | Zendy; Muriel Mafura | Zendy; Peter Slickers | Zendy; Karin Ballmer | Zendy; Peter Kuhnert | Zendy; Martin J. Woodward | Zendy; Ralf Ehricht | Zendy

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Pathotyping Escherichia coli by Using Miniaturized DNA Microarrays

Author(s) -

Muna F. Anjum,

Muriel Mafura,

Peter Slickers,

Karin Ballmer,

Peter Kuhnert,

Martin J. Woodward,

Ralf Ehricht

Publication year - 2007

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00419-07

Subject(s) - escherichia coli , virulence , dna microarray , biology , oligonucleotide , microarray , microbiology and biotechnology , pathogenic escherichia coli , dna , genetics , computational biology , gene , gene expression

The detection of virulence determinants harbored by pathogenic Escherichia coli is important for establishing the pathotype responsible for infection. A sensitive and specific miniaturized virulence microarray containing 60 oligonucleotide probes was developed. It detected six E. coli pathotypes and will be suitable in the future for high-throughput use.

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