Identification and Characterization of Bacillus anthracis Spores by Multiparameter Flow Cytometry
Author(s) -
William C. Schumacher,
Craig A. Storozuk,
Prabir K. Dutta,
Andrew J. Phipps
Publication year - 2008
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00369-08
Subject(s) - bacillus anthracis , virulence , spore , microbiology and biotechnology , anthrax toxin , flow cytometry , biology , bacillus (shape) , plasmid , bacteria , gene , genetics , recombinant dna , fusion protein
In response to the need for methods that can rapidly detect potentially virulentBacillus anthracis spores, we developed a two-color flow cytometric assay capable of simultaneously identifyingB. anthracis spores and the presence of spore-associated protective antigen, a virulence marker for strains harboring the pXO1 plasmid.
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