Comparison of the Specificities and Efficacies of Primers for Aromatic Dioxygenase Gene Analysis of Environmental Samples | Zendy

Shoko Iwai | Zendy; Timothy A. Johnson | Zendy; Benli Chai | Zendy; Syed A. Hashsham | Zendy; James M. Tiedje | Zendy

Open Access

Comparison of the Specificities and Efficacies of Primers for Aromatic Dioxygenase Gene Analysis of Environmental Samples

Author(s) -

Shoko Iwai,

Timothy A. Johnson,

Benli Chai,

Syed A. Hashsham,

James M. Tiedje

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00331-11

Subject(s) - dioxygenase , in silico , primer (cosmetics) , gene , biology , computational biology , bioremediation , genetics , chemistry , bacteria , organic chemistry

Aromatic dioxygenase genes have long been of interest for bioremediation and aromatic carbon cycling studies. To date, 115 primers and probes have been designed and used to analyze dioxygenase gene diversities in environmental samples. Here we analyze those primers' specificities, coverages, and PCR product lengths compared to known aromatic dioxygenase genes based onin silico analysis as well as summarize their differing advantages or effectiveness from over 50 reported experimental studies. We also provide some guidance for primer use in future studies.

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