Deletion Mutant Library for Investigation of Functional Outputs of Cyclic Diguanylate Metabolism in Pseudomonas aeruginosa PA14 | Zendy

Dae-Gon Ha | Zendy; Megan E. Richman | Zendy; George A. O’Toole | Zendy

Open Access

Deletion Mutant Library for Investigation of Functional Outputs of Cyclic Diguanylate Metabolism in Pseudomonas aeruginosa PA14

Author(s) -

Dae-Gon Ha,

Megan E. Richman,

George A. O’Toole

Publication year - 2014

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00299-14

Subject(s) - pseudomonas aeruginosa , mutant , swarming motility , motility , biofilm , swarming (honey bee) , biology , gene , microbiology and biotechnology , biochemistry , computational biology , bacteria , quorum sensing , genetics

We constructed a library of in-frame deletion mutants targeting each gene inPseudomonas aeruginosa PA14 predicted to participate in cyclic di-GMP (c-di-GMP) metabolism (biosynthesis or degradation) to provide a toolkit to assist investigators studying c-di-GMP-mediated regulation by this microbe. We present phenotypic assessments of each mutant, including biofilm formation, exopolysaccharide (EPS) production, swimming motility, swarming motility, and twitch motility, as a means to initially characterize these mutants and to demonstrate the potential utility of this library.

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