Membrane-Bound Pyrroloquinoline Quinone-Dependent Dehydrogenase in Gluconobacter oxydans M5, Responsible for Production of 6-(2-Hydroxyethyl) Amino-6-Deoxy- l -Sorbose | Zendy

Xuepeng Yang | Zendy; Liujing Wei | Zendy; Jinping Lin | Zendy; Bo Yin | Zendy; Dongzhi Wei | Zendy

Open Access

Membrane-Bound Pyrroloquinoline Quinone-Dependent Dehydrogenase in Gluconobacter oxydans M5, Responsible for Production of 6-(2-Hydroxyethyl) Amino-6-Deoxy- l -Sorbose

Author(s) -

Xuepeng Yang,

Liujing Wei,

Jinping Lin,

Bo Yin,

Dongzhi Wei

Publication year - 2008

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00272-08

Subject(s) - pyrroloquinoline quinone , biochemistry , sorbose , sorbitol dehydrogenase , chemistry , dehydrogenase , sorbitol , enzyme , cofactor , fructose

A membrane-bound protein purified from Gluconobacter oxydans M5 was confirmed to be a pyrroloquinoline quinone-dependent D-sorbitol dehydrogenase. Gene disruption and complementation experiments demonstrated that this enzyme is responsible for the oxidation of 1-(2-hydroxyethyl) amino-1-deoxy-D-sorbitol (1NSL) to 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose (6NSE), which is the precursor of an antidiabetic drug, miglitol.

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