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A multiplex PCR was designed to detect Escherichia coli O104:H4, a hybrid pathotype of Shiga toxigenic and enteroaggregative E. coli, in cattle feces. A total of 248 fecal samples were tested, and 20.6% were positive for serogroup O104. The O104 isolates did not carry genes characteristic of the virulent hybrid strain.

applied and environmental microbiology

Detection of Escherichia coli O104 in the Feces of Feedlot Cattle by a Multiplex PCR Assay Designed To Target Major Genetic Traits of the Virulent Hybrid Strain Responsible for the 2011 German Outbreak