Detection of Escherichia coli O104 in the Feces of Feedlot Cattle by a Multiplex PCR Assay Designed To Target Major Genetic Traits of the Virulent Hybrid Strain Responsible for the 2011 German Outbreak | Zendy

Z. D. Paddock | Zendy; Jianfa Bai | Zendy; Xiaorong Shi | Zendy; David G. Renter | Zendy; T. G. Nagaraja | Zendy

Open Access

Detection of Escherichia coli O104 in the Feces of Feedlot Cattle by a Multiplex PCR Assay Designed To Target Major Genetic Traits of the Virulent Hybrid Strain Responsible for the 2011 German Outbreak

Author(s) -

Z. D. Paddock,

Jianfa Bai,

Xiaorong Shi,

David G. Renter,

T. G. Nagaraja

Publication year - 2013

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00246-13

Subject(s) - biology , feces , virulence , escherichia coli , multiplex polymerase chain reaction , multiplex , microbiology and biotechnology , strain (injury) , polymerase chain reaction , gene , genetics , anatomy

A multiplex PCR was designed to detect Escherichia coli O104:H4, a hybrid pathotype of Shiga toxigenic and enteroaggregative E. coli, in cattle feces. A total of 248 fecal samples were tested, and 20.6% were positive for serogroup O104. The O104 isolates did not carry genes characteristic of the virulent hybrid strain.

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