Open AccessUse of a Mixture of Surrogates for Infectious Bioagents in a Standard Approach to Assessing Disinfection of Environmental Surfaces
Author(s) -
Safaa Sabbah,
Susan Springthorpe,
Syed A. Sattar
Publication year - 2010
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00246-10
Subject(s) - environmental science , water disinfection , biochemical engineering , environmental engineering , engineering
We used a mixture of surrogates (Acinetobacter baumannii ,Mycobacterium terrae , hepatitis A virus, and spores ofGeobacillus stearothermophilus ) for bioagents in a standardized approach to test environmental surface disinfectants. Each carrier containing 10 μl of mixture received 50 μl of a test chemical or saline at 22 ± 2°C. Disinfectant efficacy criteria were ≥6 log10 reduction for the bacteria and the spores and ≥3 log10 reduction for the virus. Peracetic acid (1,000 ppm) was effective in 5 min against the two bacteria and the spores but not against the virus. Chlorine dioxide (CD; 500 and 1,000 ppm) and domestic bleach (DB; 2,500, 3,500, and 5,000 ppm) were effective in 5 min, except for sporicidal activity, which needed 20 min of contact with either 1,000 ppm of CD or the two higher concentrations of DB.