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Insertion sequences play important roles in bacterial evolution and are frequently utilized in mutagenesis systems. However, the intrinsic insertion sequences of tetragenococci are not well characterized. Here, we identified three active insertion sequences ofT. halophilus by transposition into the region around thearc operon. This report provides an example of insertion sequence-mediated generation and evolution ofT. halophilus and primary information about their characteristics.

applied and environmental microbiology

Transposition of IS 4 Family Insertion Sequences IS Teha3 , IS Teha4 , and IS Teha5 into the arc Operon Disrupts Arginine Deiminase System in Tetragenococcus halophilus