Open AccessGlycerol Utilization Gene Cluster in Streptomyces clavuligerus
Author(s) -
Sonia Baños,
Rosario PérezRedondo,
Bert Koekman,
Paloma Liras
Publication year - 2009
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00181-09
Subject(s) - streptomyces clavuligerus , glycerol , clavulanic acid , gene cluster , biology , biochemistry , strain (injury) , mutant , streptomyces , streptomycetaceae , gene , actinomycetales , chemistry , bacteria , genetics , antibiotics , anatomy , amoxicillin
TheStreptomyces clavuligerus ATCC 27064 glycerol clustergylR-glpF1K1D1 is induced by glycerol but is not affected by glucose.S. clavuligerus growth and clavulanic acid production are stimulated by glycerol, but this does not occur in aglpK1 -deleted mutant. Amplification ofglpK1D1 results in transformants yielding larger amounts of clavulanic acid in the wild-type strain and in overproducerS. clavuligerus Gap15-7-30 orS. clavuligerus ΔrelA strains.