In Vivo Transduction of an Stx-Encoding Phage in Ruminants | Zendy

Nancy A. Cornick | Zendy; Amy F. Helgerson | Zendy; Volker Mai | Zendy; Jennifer M. Ritchie | Zendy; David W. K. Acheson | Zendy

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In Vivo Transduction of an Stx-Encoding Phage in Ruminants

Author(s) -

Nancy A. Cornick,

Amy F. Helgerson,

Volker Mai,

Jennifer M. Ritchie,

David W. K. Acheson

Publication year - 2006

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00157-06

Subject(s) - transduction (biophysics) , kanamycin , biology , escherichia coli , enrofloxacin , microbiology and biotechnology , in vivo , gastrointestinal tract , quinolone , antibiotics , genetics , gene , biochemistry , ciprofloxacin

We assessed the ability of a kanamycin-marked Stx phage to move into a commensal, ovine Escherichia coli strain in the ruminant gastrointestinal tract. Transduction was detected in 19/24 sheep tested, resulting in the recovery of 47 transductants. Subtherapeutic doses of the quinolone antibiotic enrofloxacin did not increase the rate of transduction.

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