Development of a Reverse Transcription-Quantitative PCR Assay for Detection of Salivirus/Klassevirus | Zendy

Eiji Haramoto | Zendy; Masaaki Kitajima | Zendy; Mikie Otagiri | Zendy

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Development of a Reverse Transcription-Quantitative PCR Assay for Detection of Salivirus/Klassevirus

Author(s) -

Eiji Haramoto,

Masaaki Kitajima,

Mikie Otagiri

Publication year - 2013

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00132-13

Subject(s) - sewage , reverse transcription polymerase chain reaction , chromatography , reverse transcriptase , real time polymerase chain reaction , biology , microbiology and biotechnology , chemistry , polymerase chain reaction , environmental science , genetics , gene , gene expression , environmental engineering

A broadly reactive and highly sensitive reverse transcription-quantitative PCR assay to detect salivirus/klassevirus was developed. By means of the developed assay, salivirus/klassevirus was detected in 13 (93%) raw sewage, 4 (29%) secondary-treated sewage, and 9 (16%) river water samples, with a maximum concentration of 9.7 × 10(6) copies/liter.

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