Open AccessComparison of Genotypic and Phylogenetic Relationships of Environmental Enterococcus Isolates by BOX-PCR Typing and 16S rRNA Gene Sequencing
Author(s) -
Biyak,
Brian D. Badgley,
Valerie J. Harwood
Publication year - 2011
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00130-11
Subject(s) - biology , phylogenetic tree , genetics , 16s ribosomal rna , typing , enterococcus , genotype , gene , multilocus sequence typing , phylogenetics , ribosomal rna , polymerase chain reaction , bacteria
EnvironmentalEnterococcus spp. were compared by BOX-PCR genotyping and 16S rRNA gene sequencing to clarify the predictive relationship of BOX-PCR fingerprints to species designation. BOX-PCR and 16S rRNA gene relationships agreed for 77% of strains. BOX-PCR provided superior intraspecies discrimination but incorrectly identified some strains to the species level and divided some species into multiple groups.