Rapid and Sensitive Detection of Norovirus Genomes in Oysters by a Two-Step Isothermal Amplification Assay System Combining Nucleic Acid Sequence-Based Amplification and Reverse Transcription-Loop-Mediated Isothermal Amplification Assays | Zendy

Shinji Fukuda | Zendy; Yukie Sasaki | Zendy; Masato Seno | Zendy

Open Access

Rapid and Sensitive Detection of Norovirus Genomes in Oysters by a Two-Step Isothermal Amplification Assay System Combining Nucleic Acid Sequence-Based Amplification and Reverse Transcription-Loop-Mediated Isothermal Amplification Assays

Author(s) -

Shinji Fukuda,

Yukie Sasaki,

Masato Seno

Publication year - 2008

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00127-08

Subject(s) - loop mediated isothermal amplification , reverse transcription loop mediated isothermal amplification , biology , recombinase polymerase amplification , nucleic acid , reverse transcriptase , genome , microbiology and biotechnology , multiple displacement amplification , norovirus , rna , polymerase chain reaction , virology , gene , genetics , dna , virus , dna extraction

We developed a two-step isothermal amplification assay system, which achieved the detection of norovirus (NoV) genomes in oysters with a sensitivity similar to that of reverse transcription-seminested PCR. The time taken for the amplification of NoV genomes from RNA extracts was shortened to about 3 h.

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