Shotgun Isotope Array for Rapid, Substrate-Specific Detection of Microorganisms in a Microbial Community | Zendy

Tomohiro Tobino | Zendy; Futoshi Kurisu | Zendy; Ikuro Kasuga | Zendy; Hiroaki Furumai | Zendy

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Shotgun Isotope Array for Rapid, Substrate-Specific Detection of Microorganisms in a Microbial Community

Author(s) -

Tomohiro Tobino,

Futoshi Kurisu,

Ikuro Kasuga,

Hiroaki Furumai

Publication year - 2011

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00121-11

Subject(s) - microorganism , biology , substrate (aquarium) , microbial population biology , bacteria , microbiology and biotechnology , chemistry , environmental chemistry , ecology , genetics

The shotgun isotope array method has been proposed to be an effective new tool for use in substrate-specific microbe exploration without any prior knowledge of the community composition. Proof of concept was demonstrated by detection of acetate-degrading microorganisms in activated sludge and further verified by independent stable isotope probing (SIP).

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