Open AccessQuantitative Real-Time PCR Assays for Sensitive Detection of Canada Goose-Specific Fecal Pollution in Water Sources
Author(s) -
B. Frémaux,
Tyler Boa,
Christopher K. Yost
Publication year - 2010
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00110-10
Subject(s) - goose , branta , feces , biology , 16s ribosomal rna , bacteroides , bacteroidaceae , fecal coliform , pollution , microbiology and biotechnology , zoology , ecology , water quality , gene , bacteria , genetics
Canada geese (Branta canadensis ) are prevalent in North America and may contribute to fecal pollution of water systems where they congregate. This work provides two novel real-time PCR assays (CGOF1-Bac and CGOF2-Bac ) allowing for the specific and sensitive detection ofBacteroides 16S rRNA gene markers present within Canada goose feces.