The Basic-Region Helix-Loop-Helix Transcription Factor DevR Significantly Affects Polysaccharide Metabolism in Aspergillus oryzae | Zendy

Zhuang Miao | Zendy; Zhimin Zhang | Zendy; Long Jin | Zendy; BaoTeng Wang | Zendy; Yasuji Koyama | Zendy; FengJie Jin | Zendy

Open Access

The Basic-Region Helix-Loop-Helix Transcription Factor DevR Significantly Affects Polysaccharide Metabolism in Aspergillus oryzae

Author(s) -

Zhuang Miao,

Zhimin Zhang,

Long Jin,

BaoTeng Wang,

Yasuji Koyama,

FengJie Jin

Publication year - 2019

Publication title -

applied and environmental microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.552

H-Index - 324

eISSN - 1070-6291

pISSN - 0099-2240

DOI - 10.1128/aem.00089-19

Subject(s) - aspergillus oryzae , conidiation , aspergillus nidulans , biology , transcription factor , microbiology and biotechnology , fungal protein , aspergillus fumigatus , biochemistry , gene , saccharomyces cerevisiae , enzyme , virulence , mutant

Aspergillus oryzae is an industrially important filamentous fungus; therefore, a clear understanding of its polysaccharide metabolism and utilization is very important for its industrial utilization. In this study, we revealed that the basic-region helix-loop-helix (bHLH) transcription factor AoDevR is importantly involved in chitin and starch metabolism inA. oryzae . The overexpression of AodevR strongly suppressed the expression of amylase-related genes. The results of a yeast one-hybrid assay suggested that the DevR protein potentially interacts with the promoter ofamyR , which encodes a transcription factor involved in amylase production and starch utilization. This study provides new insight for further revealing the regulation mechanism of amylase production inA. oryzae .

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