Open AccessChlorine Sensitivity of Feline Calicivirus, a Norovirus Surrogate
Author(s) -
Hiroshi Urakami,
Kumiko Ikarashi,
Okamoto Ko,
Yukari Abe,
Tamami Ikarashi,
Takeshi Kono,
Yukifumi Konagaya,
Nobuaki Tanaka
Publication year - 2007
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00083-07
Subject(s) - feline calicivirus , infectivity , chlorine , sodium hypochlorite , norovirus , calicivirus , murine norovirus , chemistry , virology , trichloroisocyanuric acid , microbiology and biotechnology , biology , virus , organic chemistry
The sensitivity to free chlorine of feline calicivirus (FCV), a norovirus surrogate, was examined relative to chlorine demand. When a crude suspension of FCV was treated with a sodium hypochlorite solution containing 10 μg/ml free chlorine, the extent of the decrease of viral infectivity clearly depended on the volume of the reaction mixture. The apparent sensitivity of FCV to free chlorine increased with the reduction of host cell debris, indicating that chlorine demand must be minimized to know the true sensitivity of the virus. We therefore partially purified the viruses from the host cell components and found that the infectivity of FCV was reduced by more than log 4.6 by 5 min of treatment with 300 ng/ml free chlorine.