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Plasmid-Borne Type E Neurotoxin Gene Clusters in Clostridium botulinum Strains
Author(s) -
Zhen Zhang,
Hannamari Hintsa,
Ying Chen,
Hannu Korkeala,
Miia Lindstrï¿ ⁄ m
Publication year - 2013
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00080-13
Subject(s) - clostridium botulinum , neurotoxin , pulsed field gel electrophoresis , plasmid , clostridiaceae , biology , microbiology and biotechnology , gene cluster , gene , clostridium , botulism , gel electrophoresis , bacteria , genetics , toxin , genotype , biochemistry
A collection of 36Clostridium botulinum type E strains was examined by pulsed-field gel electrophoresis (PFGE) and Southern hybridization with probes targeted tobotE andorfX1 in the neurotoxin gene cluster. Three strains were found to contain neurotoxin subtype E1 gene clusters in large plasmids of about 146 kb in size.

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