Open AccessIsolation of VanA-Type Vancomycin-Resistant Enterococcus Strains from Domestic Poultry Products with Enrichment by Incubation in Buffered Peptone Water at 42°C
Author(s) -
Tetsuya Harada,
Masashi Kanki,
Takao Kawai,
Masumi Taguchi,
Tsutomu Asao,
Yuko Kumeda
Publication year - 2010
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00071-10
Subject(s) - incubation , enterococcus faecalis , microbiology and biotechnology , enterococcus , isolation (microbiology) , biology , clone (java method) , incubation period , bacteria , streptococcaceae , staphylococcus aureus , antibiotics , biochemistry , dna , genetics
Eight VanA-type enterococcal strains were isolated from 8 of 171 domestic poultry products by using enrichment by incubation in buffered peptone water at 35°C and 42°C. The pulsed-field gel electrophoresis patterns of all six VanA-typeEnterococcus faecalis isolates were nearly indistinguishable, indicating the presence of a specific clone in Japan.