Open AccessEnhancement of Targeted Homologous Recombination inMycoplasma mycoidessubsp.capriby Inclusion of HeterologousrecA
Author(s) -
Ayman B. Allam,
Leticia Reyes,
Nacyra Assad-Garcia,
John I. Glass,
Mary B. Brown
Publication year - 2010
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00056-10
Subject(s) - heterologous , mycoplasma mycoides , biology , homologous recombination , homologous chromosome , microbiology and biotechnology , recombination , mycoplasma , virology , genetics , dna , gene
A suicide plasmid, pExp1-ctpA::tetM-recAec, employing recA from Escherichia coli and tetM as a selection marker, was used to generate ctpA knockout mutants in Mycoplasma mycoides subsp. capri through targeted gene disruption. Inclusion of E. coli recA greatly enhanced both the consistency and the recovery of mutants generated by homologous recombination.
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