Open AccessEffect of Soil Clay Content on RNA Isolation and on Detection and Quantification of Bacterial Gene Transcripts in Soil by Quantitative Reverse Transcription-PCR
Author(s) -
Amy Novinscak,
Martin Filion
Publication year - 2011
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00055-11
Subject(s) - rna extraction , reverse transcription polymerase chain reaction , gene , rna , biology , transcription (linguistics) , reverse transcriptase , isolation (microbiology) , clay soil , real time polymerase chain reaction , gene expression , microbiology and biotechnology , soil water , genetics , ecology , linguistics , philosophy
In this study, we evaluated the effect of soil clay content on RNA isolation and on quantitative reverse transcription-PCR (qRT-PCR) quantification of microbial gene transcripts. The amount of clay significantly altered RNA isolation yields and qRT-PCR analyses. Recommendations are made for quantifying microbial gene transcripts in soil samples varying in clay content.