Open AccessHollow-Fiber Ultrafiltration and PCR Detection of Human-Associated Genetic Markers from Various Types of Surface Water in Florida
Author(s) -
Stephaney D. Leskinen,
Miriam Brownell,
Daniel V. Lim,
Valerie J. Harwood
Publication year - 2010
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00025-10
Subject(s) - ultrafiltration (renal) , filtration (mathematics) , fiber , genetic marker , biology , membrane , chromatography , polymerase chain reaction , surface water , chemistry , gene , genetics , environmental science , environmental engineering , statistics , mathematics , organic chemistry
Hollow-fiber ultrafiltration (HFUF) and PCR were combined to detect human-associated microbial source tracking marker genes in large volumes of fresh and estuarine Florida water. HFUF allowed marker detection when membrane filtration did not, demonstrating HFUF's ability to facilitate detection of diluted targets by PCR in a variety of water types.