Open AccessCell-FreeEscherichia coli-Based System To Screen for Quorum-Sensing Molecules Interacting with Quorum Receptor Proteins ofStreptomyces coelicolor
Author(s) -
YungHun Yang,
TaeWan Kim,
Sung-Hee Park,
Kwang Won Lee,
Hyung-Yeon Park,
Eun Kee Song,
Hwang-Soo Joo,
YunGon Kim,
Jinyong Hahn,
ByungGee Kim
Publication year - 2009
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.00019-09
Subject(s) - streptomyces coelicolor , quorum sensing , escherichia coli , streptomyces , biology , biofilm , virulence , biochemistry , bacteria , secondary metabolism , microbiology and biotechnology , gene , biosynthesis , genetics
Quorum sensing (QS) is mediated by small molecules and involved in diverse cellular functions, such as virulence, biofilm formation, secondary metabolism, and cell differentiation. In this study, we developed a rapid and effective screening tool based on a cell-freeEscherichia coli -based expression system to identify QS molecules ofStreptomyces . The binding of QS molecules to γ-butyrolactone receptor ScbR was monitored by changes in the expression levels of the green fluorescent protein reporter inE. coli cell extract. Using this assay system, we could successfully confirm SCB1, a γ-butyrolactone molecule inStreptomyces coelicolor , binding to its known receptor, ScbR. In addition, we have shown thatN -hexanoyl-dl -homoserine lactone, one of the QS molecules in many gram-negative bacteria, can regulate ScbR and trigger precocious antibiotic production inS. coelicolor . Our new method can be applied to other strains for which a screening tool for QS molecules has not yet been developed.