Open AccessPenicillin-Induced Lysis of Escherichia coli in Medium That Does Not Support Sustained Growth
Author(s) -
Claire M. Berg,
Scott V. Anderson
Publication year - 1976
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.9.4.713
Subject(s) - lysis , escherichia coli , penicillin , microbiology and biotechnology , auxotrophy , metabolite , intracellular , enzyme , chemistry , enterobacteriaceae , antibiotics , biochemistry , biology , gene
Exponentially growing cells of anEscherichia coli auxotroph that are washed and resuspended in buffer containing a carbon source undergo sufficient growth to render them susceptible to penicillin-induced lysis, even in the absence of the required metabolite. This lysis is probably responsible for the reduction in intracellular enzyme activity previously attributed to a direct effect of penicillin upon the enzyme.