Open AccessAssociation of a Penicillin Resistance Gene with a Tetracycline Resistance Plasmid (P TP–2 ) in Staphylococcus aureus
Author(s) -
Megumi Kono,
Masanori Sasatsu
Publication year - 1976
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.9.4.706
Subject(s) - plasmid , tetracycline , microbiology and biotechnology , staphylococcus aureus , plasmid preparation , chloramphenicol , penicillin , lysogenic cycle , biology , strain (injury) , dna , transformation (genetics) , transduction (biophysics) , gene , bacteria , antibiotics , escherichia coli , genetics , bacteriophage , biochemistry , pbr322 , anatomy
On transduction with a lysogenic strain ofStraphylococcus aureus isolated from a clinical specimen and having tetracycline (TC)-penicillin (PC)-chloramphenicol (CP)-resistant plasmids, the three-drug-resistant strain was frequently obtained. By repeatedly transducing from this strain, a strain (TP-2) having stable resistance to TC and PC could be obtained. In transformation with the deoxyribonucleic acid (DNA) of TP-2 as donor, all of the transformants obtained by selecting with either TC or PC were both TC and PC resistant. According to electron microscopy study of the covalently closed circular DNA of TP-2, the plasmid DNA size was 1.37 ± 0.03 μm (2.84 × 106 daltons). The plasmid (PTP-2 ) is presumed to be a new plasmid in which the PC resistance gene was integrated into the TC-resistant plasmid.