Open AccessA New Actinomycin Complex Produced by a Micromonospora Species: Fermentation, Isolation, and Characterization
Author(s) -
G. H. Wagman,
Joseph A. Marquez,
P. D. Watkins,
Frank Gentile,
A. Murawski,
M. Patel,
Marvin Weinstein
Publication year - 1976
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.9.3.465
Subject(s) - micromonospora , fermentation , hydrolysis , acid hydrolysis , amino acid , isolation (microbiology) , antibiotics , biochemistry , bacteria , biology , extraction (chemistry) , paper chromatography , chemistry , chromatography , microbiology and biotechnology , streptomyces , genetics
A species ofMicromonospora, Micromonospora floridensis NRRL 8020, has been found to produce an actinomycin complex consisting of at least 25 active components. After solvent extraction of the complex, separation of the individual components was carried out by preparative thin-layer chromatography. Hydrolysis and subsequent electrophoretic and chromatographic identification of the amino acid content of each of the isolated components have shown differences from known actinomycins, and the probability exists that these contain a number of amino or imino acids not previously found in other members of this group of antibiotics.