Open AccessSimple Assay for 5-Fluorocytosine in the Presence of Amphotericin B
Author(s) -
Carol A. Kauffman,
Judith Carleton,
Peter T. Frame
Publication year - 1976
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.9.3.381
Subject(s) - amphotericin b , flucytosine , dilution , antifungal , pharmacology , biology , microbiology and biotechnology , chemistry , physics , thermodynamics
A simple method for the measurement of 5-fluorocytosine in the presence of amphotericin B is described. The antifungal activity of amphotericin B is abolished by heating serum at 100 C for 45 min. 5-Fluorocytosine is unaffected by this treatment, and serum levels can be subsequently assayed by either tube dilution or disk diffusion methods.
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