Open AccessRadioimmunoassay of Sisomicin
Author(s) -
Alan Broughton,
James E. Strong,
Gerald P. Bodey
Publication year - 1976
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.9.2.247
Subject(s) - sisomicin , radioimmunoassay , chemistry , succinimide , chromatography , conjugate , aminoglycoside , standard curve , gentamicin , antibiotics , biochemistry , mathematics , mathematical analysis , tobramycin
An antibody induced in rabbits against a gentamicin-bovine serum albumin conjugate cross-reacted with the chemically related aminoglycoside, sisomicin, facilitating the development of a sisomicin radioimmunoassay. Sisomicin was labeled with125 I using an acylating agent [3-(4-hydroxyphenyl)propionic acid-N -hydroxy-succinimide ester]. The resulting assay produced a linear standard curve on a logit-log plot with a sensitivity of 140 pg. Comparison with a microbiological assay showed no significant difference (P < 0.001) in the measurement of sisomicin by these two methods.