Radioimmunoassay of Sisomicin | Zendy

Alan Broughton | Zendy; James E. Strong | Zendy; Gerald P. Bodey | Zendy

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Radioimmunoassay of Sisomicin

Author(s) -

Alan Broughton,

James E. Strong,

Gerald P. Bodey

Publication year - 1976

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.9.2.247

Subject(s) - sisomicin , radioimmunoassay , chemistry , succinimide , chromatography , conjugate , aminoglycoside , standard curve , gentamicin , antibiotics , biochemistry , mathematics , mathematical analysis , tobramycin

An antibody induced in rabbits against a gentamicin-bovine serum albumin conjugate cross-reacted with the chemically related aminoglycoside, sisomicin, facilitating the development of a sisomicin radioimmunoassay. Sisomicin was labeled with125 I using an acylating agent [3-(4-hydroxyphenyl)propionic acid-N -hydroxy-succinimide ester]. The resulting assay produced a linear standard curve on a logit-log plot with a sensitivity of 140 pg. Comparison with a microbiological assay showed no significant difference (P < 0.001) in the measurement of sisomicin by these two methods.

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