Effect of Bleomycin on Deoxyribonucleic Acid Synthesis in Toluene-Treated Escherichia coli Cells | Zendy

Steven L. Ross | Zendy; Robb E. Moses | Zendy

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Effect of Bleomycin on Deoxyribonucleic Acid Synthesis in Toluene-Treated Escherichia coli Cells

Author(s) -

Steven L. Ross,

Robb E. Moses

Publication year - 1976

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.9.2.239

Subject(s) - bleomycin , dna synthesis , dna polymerase , escherichia coli , dna replication , dna , dna polymerase ii , microbiology and biotechnology , polymerase , dna clamp , biology , dna polymerase i , biochemistry , chemistry , polymerase chain reaction , genetics , gene , reverse transcriptase , chemotherapy

The antibiotic bleomycin stimulates deoxyribonucleic acid (DNA) synthesis in toluene-treated Escherichia coli cells. The increase in synthesis is linear with bleomycin concentration. Bleomycin-stimulated DNA synthesis is independent of replication and dependent on DNA polymerase I. Replication is spared as the DNA polymerase I-dependent DNA synthesis increases. Bleomycin does not appear to have any effect on purified E. coli DNA polymerases I or II. Our results suggest that bleomycin causes nicking of the bacterial chromosome with subsequent DNA synthesis catalyzed by DNA polymerase I.

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