Open AccessSynthesis of Vaccinia Virus Polypeptides in the Presence of Hydroxyurea
Author(s) -
Ehud Katz,
Eva Margalith,
Bela Winer
Publication year - 1974
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.6.5.647
Subject(s) - vaccinia , virus , hela , virology , biology , polyacrylamide gel electrophoresis , hydroxycarbamide , poxviridae , gel electrophoresis , dna , dna synthesis , modified vaccinia ankara , microbiology and biotechnology , cell culture , chemistry , biochemistry , genetics , chemotherapy , gene , enzyme , recombinant dna
Hydroxyurea (5 × 10−3 M) inhibits the development of vaccinia virus in HeLa cells early after infection and prevents the synthesis of viral deoxyribonucleic acid. In the presence of hydroxyurea, late viral polypeptides are not formed, as observed by electrophoresis on polyacrylamide gels.