Open AccessSynthesis of Deactoxycephalosporin C by a Mutant of Cephalosporium acremonium
Author(s) -
Stephen W. Queener,
J. J. Capone,
A. B. Radue,
Ramaswamy Nagarajan
Publication year - 1974
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.6.3.334
Subject(s) - acremonium , cephalosporin c , cephalosporin , mutant , penicillin , strain (injury) , chemistry , microbiology and biotechnology , stereochemistry , biology , biochemistry , antibiotics , botany , anatomy , gene
A number ofCephalosporium acremonium mutants blocked in the synthesis of cephalosporin C were investigated for accumulation of other β-lactam compounds. The non-cephalosporin C producers were isolated after exposing the superior cephalosporin C-producing strain M8650-4 to ultraviolet light (268 nm). One of the blocked mutants, MH63, accumulated deacetylcephalosporin C (0.4 mg/ml), deacetoxycephalosporin C (1.5 mg/ml), and penicillin N (2.7 mg/ml). In contrast, the parent of MH63 produced high levels of cephalosporin C as well as deacetylcephalosporin C (2.2 mg/ml) and penicillin N (1.0 mg/ml), but only traces of deacetoxycephalosporin C (about 0.1 mg/ml). Deacetoxycephalosporin C was isolated from the mutant strain and identified by ultraviolet light, nuclear magnetic resonance, bioactivity spectrum, and co-migration with authentic standard in three chromatography systems.