Open AccessEffect of Chelating Agents on the Susceptibility of Some Strains of Gram-Negative Bacteria to Some Antibacterial Agents
Author(s) -
Hasna Fahmima Haque,
A. D. Russell
Publication year - 1974
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.6.2.200
Subject(s) - cetrimide , ethylenediaminetetraacetic acid , chelation , benzalkonium chloride , microbiology and biotechnology , antibiotics , antibacterial activity , bacteria , pseudomonas aeruginosa , minimum inhibitory concentration , gram negative bacteria , chemistry , antibacterial agent , sarcina , gram positive bacteria , biology , chlorhexidine , biochemistry , escherichia coli , medicine , chromatography , organic chemistry , genetics , dentistry , gene
The effects of ethylenediaminetetraacetic acid and related chelating agents on the susceptibility ofPseudomonas aeruginosa and other strains of gram-negative bacteria to some β-lactam antibiotics and some non-antibiotic antibacterial agents have been studied by two methods: (i) the determination of minimal inhibitory concentrations in the presence and absence of a chelating agent; and (ii) pretreatment with a chelating agent, followed by exposure for up to 120 min at 37 C to a β-lactam drug in a nutrient medium or to the other type of antibacterial agent in a non-nutrient environment. The pretreatment technique gave the more significant results, especially with chlorhexidine, cetrimide, and benzalkonium chloride. Chelating agents that had previously been found to give a low order of activity as measured by effects on bacterial viability nevertheless appeared to induce some changes in the surface layers of the treated organisms, rendering them susceptible to sub-inhibitory concentrations of non-antibiotic drugs.