Open AccessPrevalence of First-Step Mutants among Levofloxacin-Susceptible Invasive Isolates of Streptococcus pneumoniae in the United States
Author(s) -
Mathias W. Pletz,
Ardaman Shergill,
Lesley McGee,
Bernard Beall,
Cynthia G. Whitney,
Keith P. Klugman
Publication year - 2006
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.50.4.1561-1563.2006
Subject(s) - levofloxacin , streptococcus pneumoniae , microbiology and biotechnology , ofloxacin , pneumococcal infections , quinolone , liter , biology , streptococcaceae , restriction fragment length polymorphism , ciprofloxacin , genotype , virology , medicine , antibiotics , genetics , gene , endocrinology
By use of a PCR-restriction fragment length polymorphism assay, we screened 496 levofloxacin-susceptible invasive pneumococcal strains (MIC ≤ 2 mg/liter) for quinolone resistance-determining region mutations known to confer fluoroquinolone resistance. Among those with a levofloxacin MIC of 2 mg/liter, 16.2% of isolates recovered from nursing home residents and 6.4% from non-nursing home residents had first-step mutations.