Open AccessIn Vitro Analysis of IS Ecp1B -Mediated Mobilization of Naturally Occurring β-Lactamase Gene bla CTX-M of Kluyvera ascorbata
Author(s) -
MarieFrédérique Lartigue,
Laurent Poirel,
Daniel Aubert,
Patrice Nordmann
Publication year - 2006
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.50.4.1282-1286.2006
Subject(s) - cefotaxime , nalidixic acid , enterobacteriaceae , ceftazidime , biology , transposition (logic) , escherichia coli , insertion sequence , microbiology and biotechnology , gene , plasmid , transposable element , genetics , bacteria , antibiotics , pseudomonas aeruginosa , mutant , linguistics , philosophy
ISEcp1B has been reported to be associated with and to mobilize the emerging expanded-spectrum β-lactamasebla CTX-M genes inEnterobacteriaceae . Thus, the ability of this insertion sequence to mobilize thebla CTX-M-2 gene was tested from its progenitor,Kluyvera ascorbata . Insertions of ISEcp1B upstream of thebla CTX-M-2 gene inK. ascorbata reference strain CIP7953 were first selected with cefotaxime (0.5 and 2 μg/ml). In those cases, ISEcp1B brought promoter sequences enhancingbla CTX-M-2 expression inK. ascorbata . Then, ISEcp1B -mediated mobilization of thebla CTX-M-2 gene fromK. ascorbata toEscherichia coli J53 was attempted. The transposition frequency of ISEcp1B-bla CTX-M-2 occurred at (6.4 ± 0.5) × 10−7 inE. coli . Cefotaxime, ceftazidime, and piperacillin enhanced transposition, whereas amoxicillin, cefuroxime, and nalidixic acid did not. Transposition was also enhanced when studied at 40°C.