Microbiological Agar Diffusion Assay for Metronidazole Concentrations in Serum | Zendy

Matthew E. Levison | Zendy

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Microbiological Agar Diffusion Assay for Metronidazole Concentrations in Serum

Author(s) -

Matthew E. Levison

Publication year - 1974

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.5.5.466

Subject(s) - metronidazole , clostridium perfringens , penicillin , gentamicin , agar , microbiology and biotechnology , chemistry , chromatography , cefoxitin , antibiotics , clostridium , agar plate , biology , bacteria , staphylococcus aureus , genetics

An agar diffusion method using paper disks on plates seeded with Clostridium perfringens and incubated anaerobically to assay serum levels of metronidazole is described. The lowest serum metronidazole concentration that could be measured was 0.7 mug/ml, and the average error was 4% for 5 mug/ml. This assay could measure metronidazole in the presence of gentamicin or in the presence of penicillin, with the use of penicillinase incorporated into the assay plates, or in presence of penicillin, methicillin, and cephalothin with prior exposure of the specimen to beta-lactamase.

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