Open AccessScreening Method of Agents Against the R Factor by the Use of an Hfr Made by Integrative Suppression with an R Factory
Author(s) -
Masanosuke Yoshikawa
Publication year - 1974
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.5.4.362
Subject(s) - hfr cell , escherichia coli , mutant , dnaa , inhibitory postsynaptic potential , bacteria , biology , microbiology and biotechnology , genetics , dna replication , dna , endocrinology , origin of replication , gene
By the use of an Hfr strain made by integrative suppression of a derepressed R factor, R100-1 in adnaA -ts mutant ofEscherichia coli and several other appropriate control strains with and without the R factor, a screening system was established which can be used to detect agents which are either inhibitory to the replication of the R factor and lead to its “curing” or are preferentially inhibitory to the growth of the R-carrying bacteria. The validity of this system was shown with several agents already known to be experimentally effective.