Open AccessInteraction of the Plasmid-Encoded Quinolone Resistance Protein QnrA with Escherichia coli Topoisomerase IV
Author(s) -
John Tran,
George A. Jacoby,
David C. Hooper
Publication year - 2005
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.49.7.3050-3052.2005
Subject(s) - escherichia coli , plasmid , topoisomerase , quinolone , microbiology and biotechnology , enterobacteriaceae , escherichia coli proteins , biology , dna , chemistry , antibiotics , genetics , gene
Purified QnrA blocked ciprofloxacin inhibition of topoisomerase IV, just as QnrA was previously found to prevent quinolone inhibition of DNA gyrase. With a gel displacement assay, tagged QnrA was shown to bind to topoisomerase IV and its subunits in a reaction that did not depend on the presence of DNA, quinolone, or ATP.