Open AccessMutagenesis Analysis of a Conserved Region Involved in Acetyl Coenzyme A Binding in the Aminoglycoside 6′- N -Acetyltransferase Type Ib Encoded by Plasmid pJHCMW1
Author(s) -
Atousa Pourreza,
Mavee Witherspoon,
Jessica K. Fox,
Jason Newmark,
Duyên Bui,
Marcelo E. Tolmasky
Publication year - 2005
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.49.7.2979-2982.2005
Subject(s) - acetyltransferase , aminoglycoside , mutagenesis , genetics , plasmid , biology , acetyltransferases , conserved sequence , microbiology and biotechnology , mutation , gene , peptide sequence , acetylation , antibiotics
Alanine scanning of motif A in the pJHCMW1-encoded aminoglycoside 6'-N-acetyltransferase type Ib identified amino acids important for the ability of the enzyme to confer wild-type levels of resistance to kanamycin and amikacin. The replacement of two amino acids, D117 or L120, with alanine residues resulted in complete loss of the resistance phenotype.