Open AccessSerine-to-Asparagine Substitution in the GyrA Gene Leads to Quinolone Resistance in Moxifloxacin-Exposed Chlamydia pneumoniae
Author(s) -
Jan Rupp,
Andreas Gebert,
Werner Solbach,
Matthias Maass
Publication year - 2005
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.49.1.406-407.2005
Subject(s) - moxifloxacin , quinolone , chlamydia , point mutation , microbiology and biotechnology , chlamydophila pneumoniae , biology , dna gyrase , levofloxacin , antibacterial agent , virology , gene , mutation , chlamydiaceae , antibiotics , genetics , escherichia coli
Quinolone resistance of Chlamydia pneumoniae has not been described previously. Serial subcultures of C. pneumoniae under increasing moxifloxacin concentrations (0.0125 to 6.4 mg/liter) resulted in a 256-fold MIC increase compared to moxifloxacin-naive strains. GyrA gene sequencing revealed a novel point mutation with a Ser-->Asn substitution. Subcultures under rifalazil and macrolides did not alter the respective MICs.