A Point Mutation in the 14α-Sterol Demethylase Gene cyp51A Contributes to Itraconazole Resistance in Aspergillus fumigatus | Zendy

Teresa M. Dı́az-Guerra | Zendy; Emilia Mellado | Zendy; M. Cuenca-Estrella | Zendy; J. L. Rodríguez-Tudela | Zendy

Open Access

A Point Mutation in the 14α-Sterol Demethylase Gene cyp51A Contributes to Itraconazole Resistance in Aspergillus fumigatus

Author(s) -

Teresa M. Dı́az-Guerra,

Emilia Mellado,

M. Cuenca-Estrella,

J. L. Rodríguez-Tudela

Publication year - 2004

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.48.3.1071.2004

Subject(s) - aspergillus fumigatus , itraconazole , point mutation , demethylase , sterol , microbiology and biotechnology , gene , biology , fungi imperfecti , aspergillus , mutation , genetics , antifungal , biochemistry , cholesterol , epigenetics

The genes encoding 14alpha-sterol demethylases (cyp51A and cyp51B) were analyzed in 12 itraconazole (ITC)-resistant and three ITC-susceptible clinical isolates of Aspergillus fumigatus. Six ITC-resistant strains exhibited a substitution of another amino acid for glycine at position 54, which is located at a very conserved region of the Cyp51A protein. The cyp51A gene from the A. fumigatus wild-type strain (CM-237) was replaced with the mutated cyp51A gene copy of an ITC-resistant strain (AF-72). Two transformants exhibited resistance to ITC, both of which had incorporated the mutated copy of the cyp51A gene.

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