Open Accessα-Defensin 1 (Human Neutrophil Protein 1) as an Antichemotactic Agent for Human Polymorphonuclear Leukocytes
Author(s) -
Patricia S. Grutkoski,
C. T. Graeber,
YowPin Lim,
Alfred Ayala,
Henry S. Simms
Publication year - 2003
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.47.8.2666-2668.2003
Subject(s) - tumor necrosis factor alpha , interleukin 8 , neutrophile , granulocyte , n formylmethionine leucyl phenylalanine , defensin , chemistry , immunology , cytokine , biological activity , peptide , microbiology and biotechnology , biochemistry , in vitro , biology
Medium conditioned by tumor necrosis factor alpha (TNF-alpha)-stimulated polymorphonuclear leukocytes (PMN) (CM-TNF) suppresses PMN migration. Therefore, we wished to identify the agent(s) in CM-TNF that mediated antichemotactic activity. CM-TNF was fractionated by high-performance liquid chromatography, and one fraction with antichemotactic activity contained the bactericidal protein human neutrophil protein 1 (HNP-1). We showed that HNP-1 suppresses PMN migration to formyl-methionyl-leucyl-phenylalanine but not to interleukin 8.