Open AccessRapid Detection and Estimation by Pyrosequencing of 23S rRNA Genes with a Single Nucleotide Polymorphism Conferring Linezolid Resistance in Enterococci
Author(s) -
Alistair Sinclair,
Catherine Arnold,
Neil Woodford
Publication year - 2003
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.47.11.3620-3622.2003
Subject(s) - 23s ribosomal rna , biology , pyrosequencing , linezolid , genetics , restriction fragment length polymorphism , gene , single nucleotide polymorphism , ribosomal rna , mutation , concordance , microbiology and biotechnology , genotype , bacteria , rna , staphylococcus aureus , ribosome , vancomycin
Pyrosequencing was used to detect rapidly and estimate the number of 23S rRNA genes with a G2576T mutation in 43 linezolid-resistant and -susceptible clinical isolates of enterococci. The method showed 100% concordance with PCR-restriction fragment length polymorphism for detecting isolates homozygous for either G2576 or T2576 or heterozygous for this mutation. A good correlation was found between linezolid MICs and the number of 23S rRNA gene copies carrying the mutation.