Open AccessTransmission Electron Microscopic Study of Antibiotic Action on Klebsiella pneumoniae Biofilm
Author(s) -
Jeff Zahller,
Philip S. Stewart
Publication year - 2002
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.46.8.2679-2683.2002
Subject(s) - biofilm , klebsiella pneumoniae , microbiology and biotechnology , ciprofloxacin , antibiotics , ampicillin , penetration (warfare) , bacteria , biology , escherichia coli , biochemistry , genetics , operations research , gene , engineering
The penetration of ampicillin and ciprofloxacin through biofilms formed by Klebsiella pneumoniae was confirmed by transmission electron microscopic observation of antibiotic-affected cells at the distal edge of the biofilm. Because the bacteria nevertheless survived antibiotic treatment, some protective mechanism other than inadequate penetration must have been at work in the biofilm.