Open AccessEffects of Chemical and Physical Agents on Viability and Infectivity of Encephalitozoon intestinalis Determined by Cell Culture and Flow Cytometry
Author(s) -
Maud Santillana-Hayat,
Claudine Sarfati,
Sandra Fournier,
Françoise Chau,
Raphaël Porcher,
JeanMichel Molina,
Francis Derouin
Publication year - 2002
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.46.6.2049-2051.2002
Subject(s) - infectivity , benzalkonium chloride , flow cytometry , microbiology and biotechnology , viability assay , spore , biology , encephalitozoon cuniculi , cell culture , cytometry , chemistry , virology , chromatography , microsporidia , virus , genetics
We combined tissue culture and flow cytometry to assess the activities of various temperatures, chemicals, and disinfectants on the viability and infectivity of spores of Encephalitozoon intestinalis. Surfanios and benzalkonium chloride, disinfectants currently used in the hospital, were remarkably efficient in destroying spore viability and infectivity.