Open AccessNew Mg 2+ -Dependent Oxytetracycline Resistance Determinant Tet 34 in Vibrio Isolates from Marine Fish Intestinal Contents
Author(s) -
Lisa aka,
Shinichi Suzuki
Publication year - 2002
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.46.5.1550-1552.2002
Subject(s) - biology , open reading frame , xanthine , cytosine , oxytetracycline , guanine , microbiology and biotechnology , vibrionaceae , nucleic acid sequence , amino acid , vibrio anguillarum , nucleotide , dna , biochemistry , vibrio , peptide sequence , gene , bacteria , genetics , antibiotics , enzyme
A new oxytetracycline (OTC) resistance (Otc(r)) determinant, Tet 34, was cloned from chromosomal DNA of Vibrio sp. no. 6 isolated from intestinal contents of cultured yellowtail (Seriola quinqueradiata). The transformant, containing cloned Tet 34, could grow in broth containing 25 microg of drug per ml with 10 mM MgCl2. Tet 34 encoded an open reading frame (ORF) 154 amino acids long. The amino acid sequence of the ORF was homologous to sequences of several bacterial xanthine-guanine phosphoribosyltransferases (XPRTs), which act in purine nucleotide salvage synthesis. Mg2+ binding site residues and the active site were highly conserved in XPRT and the ORF of Tet 34. The results suggest that Tet 34 encodes a new Mg2+-dependent Otc(r) mechanism.