Open AccessCharacterization of a New Extended-Spectrum β-Lactamase (TEM-87) Isolated in Proteus mirabilis during an Italian Survey
Author(s) -
Mariagrazia Perilli,
Bernardetta Segatore,
Maria Rosaria De Massis,
Nicola Franceschini,
Ciro Bianchi,
Gian María Rossolini,
Gianfranco Amicosante
Publication year - 2002
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.46.3.925-928.2002
Subject(s) - proteus mirabilis , aztreonam , ceftazidime , microbiology and biotechnology , escherichia coli , cephalosporin , proteus , minimum inhibitory concentration , biology , chemistry , bacteria , antibiotics , imipenem , genetics , pseudomonas aeruginosa , antibiotic resistance , gene
A new natural TEM derivative, named TEM-87, was identified in a Proteus mirabilis isolate from an Italian hospital. Compared to TEM-1, TEM-87 contains the following mutations: E104K, R164C, and M182T. Kinetic analysis of TEM-87 revealed extended-spectrum activity against oxyimino cephalosporins (preferentially ceftazidime) and aztreonam. Expression of blaTEM-87 in Escherichia coli decreased the host susceptibility to these drugs.