Open AccessRibosomal Mutations in Streptococcus pneumoniae Clinical Isolates
Author(s) -
Marja Pihlajamäki,
Janne Kataja,
Helena Seppälä,
John Elliot,
Maija Lein,
Pentti Huovinen,
Jari Jalava
Publication year - 2002
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.46.3.654-658.2002
Subject(s) - 23s ribosomal rna , lincosamides , biology , ribosomal rna , streptococcus pneumoniae , microbiology and biotechnology , genetics , mutation , allele , gene , bacteria , antibiotic resistance , ribosome , rna
Eleven clinical isolates of Streptococcus pneumoniae, isolated in Finland during 1996 to 2000, had an unusual macrolide resistance phenotype. They were resistant to macrolides and streptogramin B but susceptible, intermediate, or low-level resistant to lincosamides. No acquired macrolide resistance genes were detected from the strains. The isolates were found to have mutations in domain V of the 23S rRNA or ribosomal protein L4. Seven isolates had an A2059C mutation in two to four out of the four alleles encoding the 23S rRNA, two isolates had an A2059G mutation in two alleles, one isolate had a C2611G mutation in all four alleles, and one isolate had a 69GTG71-to-69TPS71 substitution in ribosomal protein L4.