Open AccessIntegron-Locatedoxa-32Gene Cassette Encoding an Extended-Spectrum Variant of OXA-2 β-Lactamase fromPseudomonas aeruginosa
Author(s) -
Laurent Poirel,
P. Gérôme,
Christophe de Champs,
J. Stéphanazzi,
Thierry Naas,
Patrice Nordmann
Publication year - 2002
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.46.2.566-569.2002
Subject(s) - integron , ceftazidime , cefotaxime , pseudomonas aeruginosa , biology , plasmid , gene , genetics , microbiology and biotechnology , gene cassette , aminoglycoside , acetyltransferase , antibiotics , bacteria , acetylation
Pseudomonas aeruginosa clinical isolate CY-1, which was resistant to ceftazidime, harbored a conjugative ca. 250-kb plasmid that contained a class 1 integron with two gene cassettes encoding OXA-32, an OXA-2- type beta-lactamase, and the aminoglycoside acetyltransferase AAC(6')Ib(9). OXA-32 differed from OXA-2 by an Leu169Ile amino acid substitution (class D numbering). Site-directed mutagenesis established that Ile169 is responsible for resistance to ceftazidime but not to cefotaxime.